Electron microscopic analysis of partially replicated bacteriophage T7 DNA.
نویسندگان
چکیده
منابع مشابه
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
A chemically modified phage T7 DNA polymerase has three properties that make it ideal for DNA sequencing by the chain-termination method. The enzyme is highly processive, catalyzing the polymerization of thousands of nucleotides without dissociating. By virtue of the modification the 3' to 5' exonuclease activity is eliminated. The modified polymerase efficiently uses nucleotide analogs that in...
متن کاملBacteriophage T7 DNA polymerase – sequenase
An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuclease activity; and (4) production of clear and uniform signals for detection. The DNA polymerase...
متن کاملBacteriophage T7 DNA replication. An electron microscopic study of the growing point and the role of the T7 gene 4 protein in the formation of DNA fragments.
T7 gene 4 codes for a protein whose function is essential for the replication of the viral chromosome. The experiments reported in this paper were designed to study the mechanism of action of T7 gene 4 by assessing the change that occurs in intracellular viral DNA when the gene 4 protein is inactivated during a normal DNA replication cycle. Following infection with phage carrying a temperature-...
متن کاملElectron microscopic studies of bacteriophage M13 DNA replication.
Intracellular forms of M13 phage DNA isolated after infection of Escherichia coli with wild-type phage have been studied by electron microscopy and ultracentrifugation. The data indicate the involvement of rolling-circle intermediates in single-stranded DNA synthesis. In addition to single-stranded circular DNA, we observed covalently closed and nicked replicative-form (RF) DNAs, dimer RF DNAs,...
متن کاملOrder of injection of T7 bacteriophage DNA.
The deoxyribonucleic acid (DNA) corresponding to the left-hand end of the genetic map is injected first in T7 phage infection. The order was deduced by use of marker rescue experiments to see which genes are still injected after the double-stranded DNA chain is broken by X rays. The treatment causes a selective loss of genes located on the right side of the map. Presumably, this corresponds to ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.32.2.606-613.1979